Thesis (Ph.D.) - University of Warwick, 1999.
|The Physical Object|
|Number of Pages||225|
Induction of the murine Brachyury promoter. Author: Ingram, Damien ISNI: Awarding Body: University of Warwick Current Institution: University of Warwick Date of Award: Availability of Full Text: Full text unavailable from EThOS. Buy Characterisation of the primitive streak promoter of the murine Brachyury gene by Hazel Taylor (ISBN:) from Amazon's Book Store. Everyday low prices and free delivery on eligible : Hazel Taylor. expressed under the control of the murine PGK-promoter. Expression of Brachyury is indicated (triangle). seems to be suff icient for Brachyury induction in C3H10T1/2. The transcriptional regulatory elements which control Brachyury expression in the streak and in the node-derived axial mesoderm are separable, allowing speCUlation as to the nature of the mechanisms responsible for induction of the two types of mesoderm in the murine epiblast. Brachyury homologues have been cloned from several vertebrate.
Brachyury is a T-box transcription factor that has recently been identified as a driver of the EMT process in human carcinoma cells. Overexpression of brachyury in epithelial cancer cells has been shown to drive the acquisition of a mesenchymal-like tumor phenotype, a more migratory and invasive phenotype, and a greater resistance to. (D) The two most proximal Ntl-binding sites are essential for cyp26a1 induction by Ntl. The graph shows the fold induction of Pcyp26a1:luciferase by Ntl for each of the constructs shown in C. (E) A minimum of five Ntl-binding sites with a P -value of. Brachyury (from Greek βραχύς, "short" and ουρά, "tail") is a protein that, in humans, is encoded by the TBXT (T-box transcription factor T) gene. Brachyury functions as a transcription factor within the T-box family of genes. Brachyury homologs have been found in all bilaterian animals that have been screened, as well as the freshwater cnidarian Hydra. Brachyury Review Article. From Notochord Formation to Hereditary Chordoma: The Many Roles of Brachyury; Brachyury in Chordoma. Brachyury A gene that makes a protein, also called brachyury, that is present at high levels in nearly all chordoma tumors. is highly expressed in all cells in nearly every chordoma tumor [cite source=’pubmed’][/cite].
molog of Brachyury is an immediate early response to a signal that mimics mesoderm induction in vitro, sup- ports the proposal that the Brachyury product plays a key role in the establishment and elaboration of the me- soderm and, hence, of the anteroposterior axis (Smith et al. ). The gene-regulatory mechanisms for finely balanced cell-fate determination and morphogenesis during early animal development remain largely elusive. Here, we combine genomic, single-cell imaging and biochemical approaches to chart the molecular pathways mediated by a key developmental regulator—Brachyury. Our results shed light on mechanistic insights into the ultrafine . Mesoderm induction in amphibian development is believed to involve members of the FGF and TGF- βfamilies (see Slack, ). One of the direct targets of these mesoderm-inducing factors is Xenopus Brachyury(Xbra) (Smith et al., ), a gene expressed throughout the presumptive mesoderm at the. Homologues of the murine Brachyury gene have been shown to be involved in mesoderm formation in several vertebrate species. In frogs, the Xenopus Brachyury homologue, Xbra, is required for normal formation of posterior mesoderm. We report the characterisation of a second Brachyury homologue from Xenopus, Xbra3, which has levels of identity with mouse Brachyury .